Measurement
Part:BBa_K2066028:Design
Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss Group: iGEM16_William_and_Mary (2016-10-06)
pBAD - LacI/Mcherry fusion
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1245
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1184
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1019
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1001
Design Notes
Codons were optimized for E. Coli expression and non compatible cut-sites were removed.
Source
LacI, linker, and mCherry taken from Synthetic Analog Computation in Living Cells (Daniel et al.).